ACOX1 Protein

Catalog No. ABIN4966458

This ACOX1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ACOX1 Protein (ABIN4966458)

Target: ACOX1 (Acyl-CoA Oxidase 1, Palmitoyl (ACOX1))

Origin: Sphingomonas wittichii

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Product Details

Application Details

Application Notes: Standard curve: 3 loads are recommended (0.5, 2 and 4i1/4l).For most applications a sample load of 0.2 i1/4g of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 i1/4l load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Comment:

Concentration: 0.1 pmol/μl. After re-constitution with sterile milliQ water, the final concentration of the AOX monomer is.0.1 pmol/μl. While a dimer is present in the lane, only the 27 kDa monomer contributes to the calibration.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of milliQ water. Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: ACOX1 (Acyl-CoA Oxidase 1, Palmitoyl (ACOX1))

Alternative Name: AOX (AOX)

Background: Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.Source of AOX standard: AOX standard source is Sphingomonas wittichii strain RW1, overexpressed in E.coli bearing an N-terminal his6 tag.

Molecular Weight: 27 kDa

Pathways: Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process